Construct: shRNA TRCN0000250927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081346.1-1447s21c1
DNA Barcode:
GCATCGATTCACCCGTGAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rtkn2 (170799)
Gene Description:
rhotekin 2
Transcript:
RefSeq NM_001081346.1 (CURRENT)
Match location:
Position 1447 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCGATTCACCCGTGAAAC
Hairpin Sequence:
5'-CCGG-GCATCGATTCACCCGTGAAAC-CTCGAG-GTTTCACGGGTGAATCGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCGATTCACCCGTGAAACCTCGAGGTTTCACGGGTGAATCGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCGATTCACCCGTGAAACCTCGAGGTTTCACGGGTGAATCGATGC-3'

Other clones with same target sequence:

(none)