Construct: shRNA TRCN0000250945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173393.1-1215s21c1
DNA Barcode:
TGATACTTGCCTGCCGGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xkr6 (219149)
Gene Description:
X-linked Kx blood group related 6
Transcript:
RefSeq NM_173393.1 (NON-CURRENT)
Match location:
Position 1215 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATACTTGCCTGCCGGTATT
Hairpin Sequence:
5'-CCGG-TGATACTTGCCTGCCGGTATT-CTCGAG-AATACCGGCAGGCAAGTATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATACTTGCCTGCCGGTATTCTCGAGAATACCGGCAGGCAAGTATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATACTTGCCTGCCGGTATTCTCGAGAATACCGGCAGGCAAGTATCA-3'

Other clones with same target sequence:

(none)