Construct: shRNA TRCN0000250993

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028034.2-1433s21c1
DNA Barcode:
GACATGCCAGCCCTTTCCAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tdrd12 (71981)
Gene Description:
tudor domain containing 12
Transcript:
RefSeq NM_028034.2 (CURRENT)
Match location:
Position 1433 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000250993)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACATGCCAGCCCTTTCCAAC
Hairpin Sequence:
5'-CCGG-GACATGCCAGCCCTTTCCAAC-CTCGAG-GTTGGAAAGGGCTGGCATGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACATGCCAGCCCTTTCCAACCTCGAGGTTGGAAAGGGCTGGCATGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACATGCCAGCCCTTTCCAACCTCGAGGTTGGAAAGGGCTGGCATGTC-3'

Other clones with same target sequence:

(none)