Construct: shRNA TRCN0000251000

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039710.1-1085s21c1
DNA Barcode:
TGTTGCTAGGATACCACATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Coq10b (67876)
Gene Description:
coenzyme Q10B
Transcript:
RefSeq NM_001039710.1 (CURRENT)
Match location:
Position 1085 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251000)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTGCTAGGATACCACATTC
Hairpin Sequence:
5'-CCGG-TGTTGCTAGGATACCACATTC-CTCGAG-GAATGTGGTATCCTAGCAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTGCTAGGATACCACATTCCTCGAGGAATGTGGTATCCTAGCAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTGCTAGGATACCACATTCCTCGAGGAATGTGGTATCCTAGCAACA-3'

Other clones with same target sequence:

(none)