Construct: shRNA TRCN0000251112

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146108.1-566s21c1
DNA Barcode:
GAAACGGGAATAGGACTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hibch (227095)
Gene Description:
3-hydroxyisobutyryl-Coenzyme A hydrolase
Transcript:
RefSeq NM_146108.1 (NON-CURRENT)
Match location:
Position 566 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251112)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACGGGAATAGGACTATTT
Hairpin Sequence:
5'-CCGG-GAAACGGGAATAGGACTATTT-CTCGAG-AAATAGTCCTATTCCCGTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACGGGAATAGGACTATTTCTCGAGAAATAGTCCTATTCCCGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACGGGAATAGGACTATTTCTCGAGAAATAGTCCTATTCCCGTTTC-3'

Other clones with same target sequence:

  1. TRCN0000217651