Construct: shRNA TRCN0000251129

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081391.2-12211s21c1
DNA Barcode:
TTGACTAAAGAGGTACAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csmd3 (239420)
Gene Description:
CUB and Sushi multiple domains 3
Transcript:
RefSeq NM_001081391.2 (CURRENT)
Match location:
Position 12211 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251129)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGACTAAAGAGGTACAATAT
Hairpin Sequence:
5'-CCGG-TTGACTAAAGAGGTACAATAT-CTCGAG-ATATTGTACCTCTTTAGTCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGACTAAAGAGGTACAATATCTCGAGATATTGTACCTCTTTAGTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGACTAAAGAGGTACAATATCTCGAGATATTGTACCTCTTTAGTCAA-3'

Other clones with same target sequence:

(none)