Construct: shRNA TRCN0000251171

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178640.2-366s21c1
DNA Barcode:
TTGCTGCATCATCCTACATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B3galnt2 (97884)
Gene Description:
UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 2
Transcript:
RefSeq NM_178640.2 (CURRENT)
Match location:
Position 366 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251171)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGCTGCATCATCCTACATTA
Hairpin Sequence:
5'-CCGG-TTGCTGCATCATCCTACATTA-CTCGAG-TAATGTAGGATGATGCAGCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGCTGCATCATCCTACATTACTCGAGTAATGTAGGATGATGCAGCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGCTGCATCATCCTACATTACTCGAGTAATGTAGGATGATGCAGCAA-3'

Other clones with same target sequence:

(none)