Construct: shRNA TRCN0000251183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081419.1-277s21c1
DNA Barcode:
TGACGGCAGCTACCGCTAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dip2a (64451)
Gene Description:
disco interacting protein 2 homolog A
Transcript:
RefSeq NM_001081419.1 (NON-CURRENT)
Match location:
Position 277 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACGGCAGCTACCGCTAAAC
Hairpin Sequence:
5'-CCGG-TGACGGCAGCTACCGCTAAAC-CTCGAG-GTTTAGCGGTAGCTGCCGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACGGCAGCTACCGCTAAACCTCGAGGTTTAGCGGTAGCTGCCGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACGGCAGCTACCGCTAAACCTCGAGGTTTAGCGGTAGCTGCCGTCA-3'

Other clones with same target sequence:

(none)