Construct: shRNA TRCN0000251310

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033253.2-2169s21c1
DNA Barcode:
ACCATGTCTATGATAACATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plekhg1 (213783)
Gene Description:
pleckstrin homology domain containing, family G (with RhoGef domain) member 1
Transcript:
RefSeq NM_001033253.2 (NON-CURRENT)
Match location:
Position 2169 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251310)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCATGTCTATGATAACATAA
Hairpin Sequence:
5'-CCGG-ACCATGTCTATGATAACATAA-CTCGAG-TTATGTTATCATAGACATGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCATGTCTATGATAACATAACTCGAGTTATGTTATCATAGACATGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCATGTCTATGATAACATAACTCGAGTTATGTTATCATAGACATGGT-3'

Other clones with same target sequence:

(none)