Construct: shRNA TRCN0000251323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081083.1-1436s21c1
DNA Barcode:
GCCATTGACCCGCTGATTAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Armc3 (70882)
Gene Description:
armadillo repeat containing 3
Transcript:
RefSeq NM_001081083.1 (NON-CURRENT)
Match location:
Position 1436 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTGACCCGCTGATTAAC
Hairpin Sequence:
5'-CCGG-GCCATTGACCCGCTGATTAAC-CTCGAG-GTTAATCAGCGGGTCAATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTGACCCGCTGATTAACCTCGAGGTTAATCAGCGGGTCAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTGACCCGCTGATTAACCTCGAGGTTAATCAGCGGGTCAATGGC-3'

Other clones with same target sequence:

(none)