Construct: shRNA TRCN0000251340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026854.2-2190s21c1
DNA Barcode:
GAACTTGGCTGGTCCTAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dtwd2 (68857)
Gene Description:
DTW domain containing 2
Transcript:
RefSeq NM_026854.2 (NON-CURRENT)
Match location:
Position 2190 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTTGGCTGGTCCTAATTA
Hairpin Sequence:
5'-CCGG-GAACTTGGCTGGTCCTAATTA-CTCGAG-TAATTAGGACCAGCCAAGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTTGGCTGGTCCTAATTACTCGAGTAATTAGGACCAGCCAAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTTGGCTGGTCCTAATTACTCGAGTAATTAGGACCAGCCAAGTTC-3'

Other clones with same target sequence:

(none)