Construct: shRNA TRCN0000251345

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027289.1-1068s21c1
DNA Barcode:
TGCATCACAAAGGCCCTATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nt5dc2 (70021)
Gene Description:
5'-nucleotidase domain containing 2
Transcript:
RefSeq NM_027289.1 (CURRENT)
Match location:
Position 1068 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251345)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCATCACAAAGGCCCTATTC
Hairpin Sequence:
5'-CCGG-TGCATCACAAAGGCCCTATTC-CTCGAG-GAATAGGGCCTTTGTGATGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCATCACAAAGGCCCTATTCCTCGAGGAATAGGGCCTTTGTGATGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCATCACAAAGGCCCTATTCCTCGAGGAATAGGGCCTTTGTGATGCA-3'

Other clones with same target sequence:

(none)