Construct: shRNA TRCN0000251349

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028948.1-1072s21c1
DNA Barcode:
AGACTACTGGAGGACATAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cfap53 (74453)
Gene Description:
cilia and flagella associated protein 53
Transcript:
RefSeq NM_028948.1 (NON-CURRENT)
Match location:
Position 1072 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251349)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACTACTGGAGGACATAAAG
Hairpin Sequence:
5'-CCGG-AGACTACTGGAGGACATAAAG-CTCGAG-CTTTATGTCCTCCAGTAGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACTACTGGAGGACATAAAGCTCGAGCTTTATGTCCTCCAGTAGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACTACTGGAGGACATAAAGCTCGAGCTTTATGTCCTCCAGTAGTCT-3'

Other clones with same target sequence:

(none)