Construct: shRNA TRCN0000251362

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001045530.1-795s21c1
DNA Barcode:
TTACCCTGCAAGATCATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccnjl (380694)
Gene Description:
cyclin J-like
Transcript:
RefSeq NM_001045530.1 (NON-CURRENT)
Match location:
Position 795 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251362)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACCCTGCAAGATCATATAT
Hairpin Sequence:
5'-CCGG-TTACCCTGCAAGATCATATAT-CTCGAG-ATATATGATCTTGCAGGGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACCCTGCAAGATCATATATCTCGAGATATATGATCTTGCAGGGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACCCTGCAAGATCATATATCTCGAGATATATGATCTTGCAGGGTAA-3'

Other clones with same target sequence:

(none)