Construct: shRNA TRCN0000251475

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005426.1-1137s21c1
DNA Barcode:
TCTAAGTACCACGTGACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zcwpw1 (381678)
Gene Description:
zinc finger, CW type with PWWP domain 1
Transcript:
RefSeq NM_001005426.1 (NON-CURRENT)
Match location:
Position 1137 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251475)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTAAGTACCACGTGACATTT
Hairpin Sequence:
5'-CCGG-TCTAAGTACCACGTGACATTT-CTCGAG-AAATGTCACGTGGTACTTAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTAAGTACCACGTGACATTTCTCGAGAAATGTCACGTGGTACTTAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTAAGTACCACGTGACATTTCTCGAGAAATGTCACGTGGTACTTAGA-3'

Other clones with same target sequence:

(none)