Construct: shRNA TRCN0000251528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028921.2-1642s21c1
DNA Barcode:
GCCAATTTGTTCATCCGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttll11 (74410)
Gene Description:
tubulin tyrosine ligase-like family, member 11
Transcript:
RefSeq NM_028921.2 (NON-CURRENT)
Match location:
Position 1642 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATTTGTTCATCCGATTT
Hairpin Sequence:
5'-CCGG-GCCAATTTGTTCATCCGATTT-CTCGAG-AAATCGGATGAACAAATTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATTTGTTCATCCGATTTCTCGAGAAATCGGATGAACAAATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATTTGTTCATCCGATTTCTCGAGAAATCGGATGAACAAATTGGC-3'

Other clones with same target sequence:

(none)