Construct: shRNA TRCN0000251537

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026944.1-911s21c1
DNA Barcode:
CTCCAGACAACCGAGAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Alkbh3 (69113)
Gene Description:
alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase
Transcript:
RefSeq NM_026944.1 (CURRENT)
Match location:
Position 911 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251537)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCAGACAACCGAGAGTAAA
Hairpin Sequence:
5'-CCGG-CTCCAGACAACCGAGAGTAAA-CTCGAG-TTTACTCTCGGTTGTCTGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCAGACAACCGAGAGTAAACTCGAGTTTACTCTCGGTTGTCTGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCAGACAACCGAGAGTAAACTCGAGTTTACTCTCGGTTGTCTGGAG-3'

Other clones with same target sequence:

(none)