Construct: shRNA TRCN0000251544

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033319.1-4127s21c1
DNA Barcode:
ATGTATATCCAGGCTATAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppfia1 (233977)
Gene Description:
protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
Transcript:
RefSeq NM_001033319.1 (NON-CURRENT)
Match location:
Position 4127 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251544)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTATATCCAGGCTATAAAT
Hairpin Sequence:
5'-CCGG-ATGTATATCCAGGCTATAAAT-CTCGAG-ATTTATAGCCTGGATATACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTATATCCAGGCTATAAATCTCGAGATTTATAGCCTGGATATACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTATATCCAGGCTATAAATCTCGAGATTTATAGCCTGGATATACAT-3'

Other clones with same target sequence:

  1. TRCN0000342548