Construct: shRNA TRCN0000251586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001031621.2-1833s21c1
DNA Barcode:
AGTAACTGACCACCTTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abca17 (381072)
Gene Description:
ATP-binding cassette, sub-family A (ABC1), member 17
Transcript:
RefSeq NM_001031621.2 (CURRENT)
Match location:
Position 1833 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAACTGACCACCTTTATTT
Hairpin Sequence:
5'-CCGG-AGTAACTGACCACCTTTATTT-CTCGAG-AAATAAAGGTGGTCAGTTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAACTGACCACCTTTATTTCTCGAGAAATAAAGGTGGTCAGTTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAACTGACCACCTTTATTTCTCGAGAAATAAAGGTGGTCAGTTACT-3'

Other clones with same target sequence:

(none)