Construct: shRNA TRCN0000251622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001111111.1-683s21c1
DNA Barcode:
GCTGAGCGCAATCTACGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atg16l2 (73683)
Gene Description:
autophagy related 16-like 2 (S. cerevisiae)
Transcript:
RefSeq NM_001111111.1 (CURRENT)
Match location:
Position 683 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGCGCAATCTACGTAAT
Hairpin Sequence:
5'-CCGG-GCTGAGCGCAATCTACGTAAT-CTCGAG-ATTACGTAGATTGCGCTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGCGCAATCTACGTAATCTCGAGATTACGTAGATTGCGCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGCGCAATCTACGTAATCTCGAGATTACGTAGATTGCGCTCAGC-3'

Other clones with same target sequence:

(none)