Construct: shRNA TRCN0000251681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028234.1-462s21c1
DNA Barcode:
TCTGGCATAGTTACATCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm33 (381626)
Gene Description:
RNA binding motif protein 33
Transcript:
RefSeq NM_028234.1 (CURRENT)
Match location:
Position 462 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGGCATAGTTACATCATTT
Hairpin Sequence:
5'-CCGG-TCTGGCATAGTTACATCATTT-CTCGAG-AAATGATGTAACTATGCCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGGCATAGTTACATCATTTCTCGAGAAATGATGTAACTATGCCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGGCATAGTTACATCATTTCTCGAGAAATGATGTAACTATGCCAGA-3'

Other clones with same target sequence:

(none)