Construct: shRNA TRCN0000251687

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081128.2-317s21c1
DNA Barcode:
GTATAACTCAGCCTGATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtr (238505)
Gene Description:
5-methyltetrahydrofolate-homocysteine methyltransferase
Transcript:
RefSeq NM_001081128.2 (NON-CURRENT)
Match location:
Position 317 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251687)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATAACTCAGCCTGATATTA
Hairpin Sequence:
5'-CCGG-GTATAACTCAGCCTGATATTA-CTCGAG-TAATATCAGGCTGAGTTATAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATAACTCAGCCTGATATTACTCGAGTAATATCAGGCTGAGTTATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATAACTCAGCCTGATATTACTCGAGTAATATCAGGCTGAGTTATAC-3'

Other clones with same target sequence:

(none)