Construct: shRNA TRCN0000251702

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015800.2-1739s21c1
DNA Barcode:
AGGAGAAGGACTGCGTTTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crim1 (50766)
Gene Description:
cysteine rich transmembrane BMP regulator 1 (chordin like)
Transcript:
RefSeq NM_015800.2 (NON-CURRENT)
Match location:
Position 1739 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251702)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAGAAGGACTGCGTTTATG
Hairpin Sequence:
5'-CCGG-AGGAGAAGGACTGCGTTTATG-CTCGAG-CATAAACGCAGTCCTTCTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAGAAGGACTGCGTTTATGCTCGAGCATAAACGCAGTCCTTCTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAGAAGGACTGCGTTTATGCTCGAGCATAAACGCAGTCCTTCTCCT-3'

Other clones with same target sequence:

(none)