Construct: shRNA TRCN0000251760
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001081086.1-1832s21c1
- DNA Barcode:
- AGGGATCATGAGACTACTAAA
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Ppig (228005)
- Gene Description:
- peptidyl-prolyl isomerase G (cyclophilin G)
- Transcript:
- RefSeq NM_001081086.1 (CURRENT)
- Match location:
- Position 1832 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- AGGGATCATGAGACTACTAAA
- Hairpin Sequence:
- 5'-CCGG-AGGGATCATGAGACTACTAAA-CTCGAG-TTTAGTAGTCTCATGATCCCT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGGGATCATGAGACTACTAAACTCGAGTTTAGTAGTCTCATGATCCCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGGGATCATGAGACTACTAAACTCGAGTTTAGTAGTCTCATGATCCCT-3'