Construct: shRNA TRCN0000251803

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001042421.1-6663s21c1
DNA Barcode:
ATGAAGCATCAGCGTTGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kntc1 (208628)
Gene Description:
kinetochore associated 1
Transcript:
RefSeq NM_001042421.1 (CURRENT)
Match location:
Position 6663 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251803)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAAGCATCAGCGTTGATAA
Hairpin Sequence:
5'-CCGG-ATGAAGCATCAGCGTTGATAA-CTCGAG-TTATCAACGCTGATGCTTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAAGCATCAGCGTTGATAACTCGAGTTATCAACGCTGATGCTTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAAGCATCAGCGTTGATAACTCGAGTTATCAACGCTGATGCTTCAT-3'

Other clones with same target sequence:

(none)