Construct: shRNA TRCN0000251844

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001015507.1-4505s21c1
DNA Barcode:
GCATTCAGAGGACTATGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vprbp (321006)
Gene Description:
Vpr (HIV-1) binding protein
Transcript:
RefSeq NM_001015507.1 (NON-CURRENT)
Match location:
Position 4505 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251844)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTCAGAGGACTATGATTT
Hairpin Sequence:
5'-CCGG-GCATTCAGAGGACTATGATTT-CTCGAG-AAATCATAGTCCTCTGAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTCAGAGGACTATGATTTCTCGAGAAATCATAGTCCTCTGAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTCAGAGGACTATGATTTCTCGAGAAATCATAGTCCTCTGAATGC-3'

Other clones with same target sequence:

(none)