Construct: shRNA TRCN0000251886

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030068.1-2090s21c1
DNA Barcode:
ATCCGACGCTGGGTCTTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Iqch (78250)
Gene Description:
IQ motif containing H
Transcript:
RefSeq NM_030068.1 (NON-CURRENT)
Match location:
Position 2090 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251886)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCGACGCTGGGTCTTTAAA
Hairpin Sequence:
5'-CCGG-ATCCGACGCTGGGTCTTTAAA-CTCGAG-TTTAAAGACCCAGCGTCGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCGACGCTGGGTCTTTAAACTCGAGTTTAAAGACCCAGCGTCGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCGACGCTGGGTCTTTAAACTCGAGTTTAAAGACCCAGCGTCGGAT-3'

Other clones with same target sequence:

(none)