Construct: shRNA TRCN0000251951

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026253.3-2372s21c1
DNA Barcode:
TATGACTCTACCGTCACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrc18 (67580)
Gene Description:
leucine rich repeat containing 18
Transcript:
RefSeq NM_026253.3 (NON-CURRENT)
Match location:
Position 2372 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251951)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGACTCTACCGTCACTTTA
Hairpin Sequence:
5'-CCGG-TATGACTCTACCGTCACTTTA-CTCGAG-TAAAGTGACGGTAGAGTCATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGACTCTACCGTCACTTTACTCGAGTAAAGTGACGGTAGAGTCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGACTCTACCGTCACTTTACTCGAGTAAAGTGACGGTAGAGTCATA-3'

Other clones with same target sequence:

(none)