Construct: shRNA TRCN0000251956

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027280.2-1586s21c1
DNA Barcode:
CCACACCCTAAGGCATTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nkd1 (93960)
Gene Description:
naked cuticle 1
Transcript:
RefSeq NM_027280.2 (NON-CURRENT)
Match location:
Position 1586 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000251956)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACACCCTAAGGCATTATTA
Hairpin Sequence:
5'-CCGG-CCACACCCTAAGGCATTATTA-CTCGAG-TAATAATGCCTTAGGGTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACACCCTAAGGCATTATTACTCGAGTAATAATGCCTTAGGGTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACACCCTAAGGCATTATTACTCGAGTAATAATGCCTTAGGGTGTGG-3'

Other clones with same target sequence:

(none)