Construct: shRNA TRCN0000252093

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025518.3-1253s21c1
DNA Barcode:
GAAACAGTTCAGCGAACTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dus2 (66369)
Gene Description:
dihydrouridine synthase 2
Transcript:
RefSeq NM_025518.3 (NON-CURRENT)
Match location:
Position 1253 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252093)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACAGTTCAGCGAACTATA
Hairpin Sequence:
5'-CCGG-GAAACAGTTCAGCGAACTATA-CTCGAG-TATAGTTCGCTGAACTGTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACAGTTCAGCGAACTATACTCGAGTATAGTTCGCTGAACTGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACAGTTCAGCGAACTATACTCGAGTATAGTTCGCTGAACTGTTTC-3'

Other clones with same target sequence:

(none)