Construct: shRNA TRCN0000252140

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081331.1-877s21c1
DNA Barcode:
CTGTAGTTCCCACCAGTTAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D7Ertd443e (71007)
Gene Description:
DNA segment, Chr 7, ERATO Doi 443, expressed
Transcript:
RefSeq NM_001081331.1 (CURRENT)
Match location:
Position 877 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252140)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTAGTTCCCACCAGTTAAC
Hairpin Sequence:
5'-CCGG-CTGTAGTTCCCACCAGTTAAC-CTCGAG-GTTAACTGGTGGGAACTACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTAGTTCCCACCAGTTAACCTCGAGGTTAACTGGTGGGAACTACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTAGTTCCCACCAGTTAACCTCGAGGTTAACTGGTGGGAACTACAG-3'

Other clones with same target sequence:

(none)