Construct: shRNA TRCN0000252160

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033419.2-1176s21c1
DNA Barcode:
CCAAGGACCTGCTAGTCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ceacam16 (330483)
Gene Description:
carcinoembryonic antigen-related cell adhesion molecule 16
Transcript:
RefSeq NM_001033419.2 (CURRENT)
Match location:
Position 1176 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252160)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGACCTGCTAGTCTATG
Hairpin Sequence:
5'-CCGG-CCAAGGACCTGCTAGTCTATG-CTCGAG-CATAGACTAGCAGGTCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGACCTGCTAGTCTATGCTCGAGCATAGACTAGCAGGTCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGACCTGCTAGTCTATGCTCGAGCATAGACTAGCAGGTCCTTGG-3'

Other clones with same target sequence:

(none)