Construct: shRNA TRCN0000252185

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081364.3-6311s21c1
DNA Barcode:
CCAGAAGAACACACGTATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgap21 (71435)
Gene Description:
Rho GTPase activating protein 21
Transcript:
RefSeq NM_001081364.3 (CURRENT)
Match location:
Position 6311 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252185)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAGAACACACGTATATA
Hairpin Sequence:
5'-CCGG-CCAGAAGAACACACGTATATA-CTCGAG-TATATACGTGTGTTCTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAGAACACACGTATATACTCGAGTATATACGTGTGTTCTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAGAACACACGTATATACTCGAGTATATACGTGTGTTCTTCTGG-3'

Other clones with same target sequence:

(none)