Construct: shRNA TRCN0000252222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001492.2-1596s21c1
DNA Barcode:
AGCCAATCTCACGGTCTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lca5l (385668)
Gene Description:
Leber congenital amaurosis 5-like
Transcript:
RefSeq NM_001001492.2 (CURRENT)
Match location:
Position 1596 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCAATCTCACGGTCTTAAA
Hairpin Sequence:
5'-CCGG-AGCCAATCTCACGGTCTTAAA-CTCGAG-TTTAAGACCGTGAGATTGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCAATCTCACGGTCTTAAACTCGAGTTTAAGACCGTGAGATTGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCAATCTCACGGTCTTAAACTCGAGTTTAAGACCGTGAGATTGGCT-3'

Other clones with same target sequence:

(none)