Construct: shRNA TRCN0000252247
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001081326.1-3113s21c1
- DNA Barcode:
- ACGTGAGTGGTCGGCTTATTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Agl (77559)
- Gene Description:
- amylo-1,6-glucosidase, 4-alpha-glucanotransferase
- Transcript:
- RefSeq NM_001081326.1 (CURRENT)
- Match location:
- Position 3113 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000252247)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ACGTGAGTGGTCGGCTTATTT
- Hairpin Sequence:
-
5'-CCGG-ACGTGAGTGGTCGGCTTATTT-CTCGAG-AAATAAGCCGACCACTCACGT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGACGTGAGTGGTCGGCTTATTTCTCGAGAAATAAGCCGACCACTCACGTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAACGTGAGTGGTCGGCTTATTTCTCGAGAAATAAGCCGACCACTCACGT-3'
Other clones with same target sequence:
(none)
