Construct: shRNA TRCN0000252319

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033322.1-4325s21c1
DNA Barcode:
GTATTCTCACCCTGGTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gucy1a2 (234889)
Gene Description:
guanylate cyclase 1, soluble, alpha 2
Transcript:
RefSeq NM_001033322.1 (NON-CURRENT)
Match location:
Position 4325 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252319)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATTCTCACCCTGGTATAAT
Hairpin Sequence:
5'-CCGG-GTATTCTCACCCTGGTATAAT-CTCGAG-ATTATACCAGGGTGAGAATAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATTCTCACCCTGGTATAATCTCGAGATTATACCAGGGTGAGAATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATTCTCACCCTGGTATAATCTCGAGATTATACCAGGGTGAGAATAC-3'

Other clones with same target sequence:

(none)