Construct: shRNA TRCN0000252359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080710.2-583s21c1
DNA Barcode:
TTCCTTCCTGCCATGGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sdr16c6 (242286)
Gene Description:
short chain dehydrogenase/reductase family 16C, member 6
Transcript:
RefSeq NM_001080710.2 (CURRENT)
Match location:
Position 583 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCCTTCCTGCCATGGTTAAA
Hairpin Sequence:
5'-CCGG-TTCCTTCCTGCCATGGTTAAA-CTCGAG-TTTAACCATGGCAGGAAGGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCCTTCCTGCCATGGTTAAACTCGAGTTTAACCATGGCAGGAAGGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCCTTCCTGCCATGGTTAAACTCGAGTTTAACCATGGCAGGAAGGAA-3'

Other clones with same target sequence:

(none)