Construct: shRNA TRCN0000252392

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001105245.1-3494s21c1
DNA Barcode:
GTAGTTAAGGCAGACGATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdh19 (279653)
Gene Description:
protocadherin 19
Transcript:
RefSeq NM_001105245.1 (CURRENT)
Match location:
Position 3494 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252392)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGTTAAGGCAGACGATTAT
Hairpin Sequence:
5'-CCGG-GTAGTTAAGGCAGACGATTAT-CTCGAG-ATAATCGTCTGCCTTAACTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGTTAAGGCAGACGATTATCTCGAGATAATCGTCTGCCTTAACTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGTTAAGGCAGACGATTATCTCGAGATAATCGTCTGCCTTAACTAC-3'

Other clones with same target sequence:

(none)