Construct: shRNA TRCN0000252398

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081135.1-84s21c1
DNA Barcode:
ATGCCCATGCAGTCCTGAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prrg3 (208748)
Gene Description:
proline rich Gla (G-carboxyglutamic acid) 3 (transmembrane)
Transcript:
RefSeq NM_001081135.1 (NON-CURRENT)
Match location:
Position 84 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252398)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCCCATGCAGTCCTGAAAC
Hairpin Sequence:
5'-CCGG-ATGCCCATGCAGTCCTGAAAC-CTCGAG-GTTTCAGGACTGCATGGGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCCCATGCAGTCCTGAAACCTCGAGGTTTCAGGACTGCATGGGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCCCATGCAGTCCTGAAACCTCGAGGTTTCAGGACTGCATGGGCAT-3'

Other clones with same target sequence:

(none)