Construct: shRNA TRCN0000252576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037709.1-3129s21c1
DNA Barcode:
GCCGGCTGAATGGTCAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rusc2 (100213)
Gene Description:
RUN and SH3 domain containing 2
Transcript:
RefSeq NM_001037709.1 (NON-CURRENT)
Match location:
Position 3129 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGCTGAATGGTCAGATTT
Hairpin Sequence:
5'-CCGG-GCCGGCTGAATGGTCAGATTT-CTCGAG-AAATCTGACCATTCAGCCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGCTGAATGGTCAGATTTCTCGAGAAATCTGACCATTCAGCCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGCTGAATGGTCAGATTTCTCGAGAAATCTGACCATTCAGCCGGC-3'

Other clones with same target sequence:

(none)