Construct: shRNA TRCN0000252615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153774.1-2081s21c1
DNA Barcode:
TTGACTACAGTAGTTCGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ipo9 (226432)
Gene Description:
importin 9
Transcript:
RefSeq NM_153774.1 (CURRENT)
Match location:
Position 2081 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGACTACAGTAGTTCGAAAT
Hairpin Sequence:
5'-CCGG-TTGACTACAGTAGTTCGAAAT-CTCGAG-ATTTCGAACTACTGTAGTCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGACTACAGTAGTTCGAAATCTCGAGATTTCGAACTACTGTAGTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGACTACAGTAGTTCGAAATCTCGAGATTTCGAACTACTGTAGTCAA-3'

Other clones with same target sequence:

(none)