Construct: shRNA TRCN0000252674

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081192.1-1646s21c1
DNA Barcode:
AGCGCATCCATGACTACTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hcn4 (330953)
Gene Description:
hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Transcript:
RefSeq NM_001081192.1 (CURRENT)
Match location:
Position 1646 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252674)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGCATCCATGACTACTATG
Hairpin Sequence:
5'-CCGG-AGCGCATCCATGACTACTATG-CTCGAG-CATAGTAGTCATGGATGCGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGCATCCATGACTACTATGCTCGAGCATAGTAGTCATGGATGCGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGCATCCATGACTACTATGCTCGAGCATAGTAGTCATGGATGCGCT-3'

Other clones with same target sequence:

(none)