Construct: shRNA TRCN0000252844

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027664.1-3450s21c1
DNA Barcode:
GCCACTTACATTATGATATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rimklb (108653)
Gene Description:
ribosomal modification protein rimK-like family member B
Transcript:
RefSeq NM_027664.1 (CURRENT)
Match location:
Position 3450 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252844)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACTTACATTATGATATAC
Hairpin Sequence:
5'-CCGG-GCCACTTACATTATGATATAC-CTCGAG-GTATATCATAATGTAAGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACTTACATTATGATATACCTCGAGGTATATCATAATGTAAGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACTTACATTATGATATACCTCGAGGTATATCATAATGTAAGTGGC-3'

Other clones with same target sequence:

(none)