Construct: shRNA TRCN0000252858

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144788.2-1173s21c1
DNA Barcode:
AGTAACCAGGTGTCGACAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hectd1 (207304)
Gene Description:
HECT domain E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_144788.2 (CURRENT)
Match location:
Position 1173 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252858)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAACCAGGTGTCGACAATT
Hairpin Sequence:
5'-CCGG-AGTAACCAGGTGTCGACAATT-CTCGAG-AATTGTCGACACCTGGTTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAACCAGGTGTCGACAATTCTCGAGAATTGTCGACACCTGGTTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAACCAGGTGTCGACAATTCTCGAGAATTGTCGACACCTGGTTACT-3'

Other clones with same target sequence:

(none)