Construct: shRNA TRCN0000252866

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080809.1-789s21c1
DNA Barcode:
TGTCATCCGCCTGCTAGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cps1 (227231)
Gene Description:
carbamoyl-phosphate synthetase 1
Transcript:
RefSeq NM_001080809.1 (NON-CURRENT)
Match location:
Position 789 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252866)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCATCCGCCTGCTAGTTAA
Hairpin Sequence:
5'-CCGG-TGTCATCCGCCTGCTAGTTAA-CTCGAG-TTAACTAGCAGGCGGATGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCATCCGCCTGCTAGTTAACTCGAGTTAACTAGCAGGCGGATGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCATCCGCCTGCTAGTTAACTCGAGTTAACTAGCAGGCGGATGACA-3'

Other clones with same target sequence:

(none)