Construct: shRNA TRCN0000252932

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081354.1-1240s21c1
DNA Barcode:
CTCGCCCAATGCCTTACTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mamld1 (333639)
Gene Description:
mastermind-like domain containing 1
Transcript:
RefSeq NM_001081354.1 (NON-CURRENT)
Match location:
Position 1240 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252932)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGCCCAATGCCTTACTTTC
Hairpin Sequence:
5'-CCGG-CTCGCCCAATGCCTTACTTTC-CTCGAG-GAAAGTAAGGCATTGGGCGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGCCCAATGCCTTACTTTCCTCGAGGAAAGTAAGGCATTGGGCGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGCCCAATGCCTTACTTTCCTCGAGGAAAGTAAGGCATTGGGCGAG-3'

Other clones with same target sequence:

(none)