Construct: shRNA TRCN0000252973

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033305.2-229s21c1
DNA Barcode:
CTCCAGTCTCTTCGCTGTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufb6 (230075)
Gene Description:
NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6
Transcript:
RefSeq NM_001033305.2 (NON-CURRENT)
Match location:
Position 229 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000252973)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCAGTCTCTTCGCTGTTTC
Hairpin Sequence:
5'-CCGG-CTCCAGTCTCTTCGCTGTTTC-CTCGAG-GAAACAGCGAAGAGACTGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCAGTCTCTTCGCTGTTTCCTCGAGGAAACAGCGAAGAGACTGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCAGTCTCTTCGCTGTTTCCTCGAGGAAACAGCGAAGAGACTGGAG-3'

Other clones with same target sequence:

(none)