Construct: shRNA TRCN0000253004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081395.1-971s21c1
DNA Barcode:
CAACAGTGGACAGGCGCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Amotl1 (75723)
Gene Description:
angiomotin-like 1
Transcript:
RefSeq NM_001081395.1 (CURRENT)
Match location:
Position 971 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAGTGGACAGGCGCATAA
Hairpin Sequence:
5'-CCGG-CAACAGTGGACAGGCGCATAA-CTCGAG-TTATGCGCCTGTCCACTGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAGTGGACAGGCGCATAACTCGAGTTATGCGCCTGTCCACTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAGTGGACAGGCGCATAACTCGAGTTATGCGCCTGTCCACTGTTG-3'

Other clones with same target sequence:

  1. TRCN0000420335