Construct: shRNA TRCN0000253036

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081050.2-5717s21c1
DNA Barcode:
TCGACGTAAATAGCGTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pard3b (72823)
Gene Description:
par-3 family cell polarity regulator beta
Transcript:
RefSeq NM_001081050.2 (CURRENT)
Match location:
Position 5717 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253036)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGACGTAAATAGCGTCATTT
Hairpin Sequence:
5'-CCGG-TCGACGTAAATAGCGTCATTT-CTCGAG-AAATGACGCTATTTACGTCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGACGTAAATAGCGTCATTTCTCGAGAAATGACGCTATTTACGTCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGACGTAAATAGCGTCATTTCTCGAGAAATGACGCTATTTACGTCGA-3'

Other clones with same target sequence:

(none)