Construct: shRNA TRCN0000253045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033275.4-646s21c1
DNA Barcode:
CCCAGAATTGGATGGTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gxylt1 (223827)
Gene Description:
glucoside xylosyltransferase 1
Transcript:
RefSeq NM_001033275.4 (CURRENT)
Match location:
Position 646 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAATTGGATGGTATAAT
Hairpin Sequence:
5'-CCGG-CCCAGAATTGGATGGTATAAT-CTCGAG-ATTATACCATCCAATTCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAATTGGATGGTATAATCTCGAGATTATACCATCCAATTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAATTGGATGGTATAATCTCGAGATTATACCATCCAATTCTGGG-3'

Other clones with same target sequence:

(none)